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Bioss
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mouse mmp13 elisa kit - by Bioz Stars,
2026-03
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R&D Systems
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Cusabio
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Image Search Results
Journal: Materials Today Bio
Article Title: Cuprorivaite microspheres inhibit cuproptosis and oxidative stress in osteoarthritis via Wnt/β-catenin pathway
doi: 10.1016/j.mtbio.2024.101300
Figure Lengend Snippet: Anti-inflammatory role of Cuprorivaite (CaCuSi 4 O 10 ) microspheres in IL-1β-stimulated chondrocytes. (A) Cell counting kit-8 test for cell proliferation. (B) Fluorescein diacetate for cell viability detection. (C) Cell apoptosis by flow cytometry. (D) ELISA detection for TNF-α, IL-6 and MMP13 in cell supernatant. (E) Western blot detection for TNF-α, IL-6 and MMP13 in cell lysates. (F) RT-qPCR quantification for in TNF-α, IL-6 and MMP13. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.
Article Snippet: Then, levels of TNF-α, IL-6, and MMP13 in cell supernatant and serum samples were detected by the multi-functional microplate detector, with mouse TNF-α (CSB-E04741m, CUSABIO, Wuhan, China), mouse IL-6 (CSB-E04639m, CUSABIO, Wuhan, China) and
Techniques: Cell Counting, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR
Journal: Materials Today Bio
Article Title: Cuprorivaite microspheres inhibit cuproptosis and oxidative stress in osteoarthritis via Wnt/β-catenin pathway
doi: 10.1016/j.mtbio.2024.101300
Figure Lengend Snippet: Cuprorivaite (CaCuSi 4 O 10 ) microspheres improved IL-1β-induced injury in chondrocytes via inhibiting Wnt/β-catenin pathway. (A) Cell counting kit-8 test for cell viability. (B) Fluorescein diacetate for cell viability detection. (C) Cell apoptosis by flow cytometry. (D) ELISA detection for TNF-α, IL-6 and MMP13 in cell supernatant. (E) Western blot detection for TNF-α, IL-6 and MMP13 in cell lysates. (F) RT-qPCR quantification for extracellular matrix components including collagen II and SOX9. (G) Intracellular copper content. (H) RT-qPCR quantification for cuproptosis biomarkers including ATP7B and FDX1. (I) Western blot detection for ATP7B and FDX1. (J) Oxidative stress detection including MDA, SOD and GSH. (K) RT-qPCR quantification for Wnt1, GSK3β and β-catenin. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.
Article Snippet: Then, levels of TNF-α, IL-6, and MMP13 in cell supernatant and serum samples were detected by the multi-functional microplate detector, with mouse TNF-α (CSB-E04741m, CUSABIO, Wuhan, China), mouse IL-6 (CSB-E04639m, CUSABIO, Wuhan, China) and
Techniques: Cell Counting, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR
Journal: Materials Today Bio
Article Title: Cuprorivaite microspheres inhibit cuproptosis and oxidative stress in osteoarthritis via Wnt/β-catenin pathway
doi: 10.1016/j.mtbio.2024.101300
Figure Lengend Snippet: Validation of mechanism of Cuprorivaite (CaCuSi 4 O 10 ) microspheres in OA. (A) Hematoxylin and eosin staining. (B) Safranin-O staining/fast green staining. (C) OARSI score. (D) ELISA detection for TNF-α, IL-6 and MMP13 in the serum. (E) Western blot detection for TNF-α, IL-6 and MMP13 in cartilage tissue. (F) RT-qPCR quantification for extracellular matrix components including collagen II and SOX9 in cartilage tissue. (G) Copper content in cartilage tissue. (H) RT-qPCR quantification for cuproptosis biomarkers including ATP7B and FDX1 in cartilage tissue. (I) Western blot detection for ATP7B and FDX1 in cartilage tissue. (J) Representative images of FDX1 expression in cartilage tissue detected by immunohistochemistry. (K) Oxidative stress detection including MDA, SOD and GSH in cartilage tissue. (L) RT-qPCR quantification for Wnt1, GSK3β and β-catenin in cartilage tissue. ∗∗∗ P < 0.001.
Article Snippet: Then, levels of TNF-α, IL-6, and MMP13 in cell supernatant and serum samples were detected by the multi-functional microplate detector, with mouse TNF-α (CSB-E04741m, CUSABIO, Wuhan, China), mouse IL-6 (CSB-E04639m, CUSABIO, Wuhan, China) and
Techniques: Biomarker Discovery, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR, Expressing, Immunohistochemistry